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rpl33-rps18

LSC · 71384-71615 · 232 bp

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Pi 0.0315

MarkerSeek score 69.70

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 232
variable_sites: 22
pi: 0.0315
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGACACAATACGCCTAGTCG	TCAATTCGATCCCCCGATTG	55.0	50.0	58.5	57.7	80-437 bp	1.0	82.4
rpl33-rps18_p2	GACACAATACGCCTAGTCGA	TCAATTCGATCCCCCGATTG	50.0	50.0	57.2	57.7	80-436 bp	1.0	80.0
rpl33-rps18_p3	TCGACACAATACGCCTAGTC	TCAATTCGATCCCCCGATTG	50.0	50.0	57.2	57.7	80-438 bp	1.0	80.0
rpl33-rps18_p4	CGACACAATACGCCTAGTCG	ATTCGATCCCCCGATTGGAT	55.0	50.0	58.5	58.6	80-434 bp	1.0	79.4
rpl33-rps18_p5	AGAATCGACACAATACGCCT	TCAATTCGATCCCCCGATTG	45.0	50.0	57.0	57.7	80-442 bp	1.0	79.0