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petG-trnW-CCA

LSC · 69739-69861 · 123 bp

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Pi 0.0499

MarkerSeek score 61.10

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 123
variable_sites: 13
pi: 0.0499
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACAGACGTGGTGATCAGTTG	TTCAAATCCTACAGGGCGTG	50.0	50.0	57.8	57.9	80-173 bp	1.0	88.2
petG-trnW-CCA_p2	ACAGACGTGGTGATCAGTTG	GTTCAAATCCTACAGGGCGT	50.0	50.0	57.8	57.9	80-174 bp	1.0	88.2
petG-trnW-CCA_p3	ACAGACGTGGTGATCAGTTG	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.8	58.0	80-206 bp	1.0	87.8
petG-trnW-CCA_p4	ACAGACGTGGTGATCAGTTG	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.8	57.9	80-192 bp	1.0	87.8
petG-trnW-CCA_p5	ACAGACGTGGTGATCAGTTG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	80-228 bp	1.0	87.5