Hotspot

trnR-UCU-atpA

LSC · 10232-10848 · 617 bp

Back to Photinia

Pi 0.0349

MarkerSeek score 74.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 617
variable_sites: 85
pi: 0.0349
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGAATGAAAAGCGTCCATTGT	AGACATTTACCGACGAAGCG	42.9	50.0	57.4	58.4	246-1042 bp	1.0	76.4
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	AGACATTTACCGACGAAGCG	47.6	50.0	57.3	58.4	186-982 bp	1.0	75.5
trnR-UCU-atpA_p3	TGGATAGGACAGAGGTCTTCT	AGACATTTACCGACGAAGCG	47.6	50.0	56.9	58.4	221-1017 bp	1.0	72.6
trnR-UCU-atpA_p4	GGAATGAAAAGCGTCCATTGT	GGAAGCCCTTTTGAAAGAAGC	42.9	47.6	57.4	58.0	247-1023 bp	1.0	71.1
trnR-UCU-atpA_p5	GGAATGAAAAGCGTCCATTGT	ACCAAGACATTTACCGACGA	42.9	45.0	57.4	56.9	246-1046 bp	1.0	70.5