Hotspot

rps16-trnQ-UUG

LSC · 6459-7283 · 825 bp

Back to Photinia

Pi 0.015
MarkerSeek score 60.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
825
variable_sites
53
pi
0.015
unique_haplotype_count
22
primer_available
yes
alignment_reliability
0.97
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 727-982 bp 0.962963 85.2
rps16-trnQ-UUG_p2 GCTTTCTGCCACATCGTTTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.6 58.1 704-959 bp 0.962963 83.6
rps16-trnQ-UUG_p3 CCACAGCTGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 737-992 bp 0.962963 83.5
rps16-trnQ-UUG_p4 TCCACAGCTGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 738-993 bp 0.962963 83.4
rps16-trnQ-UUG_p5 ATCCACAGCTGATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.4 58.1 739-994 bp 0.962963 81.7