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trnK-UUU-rps16

LSC · 4352-5300 · 949 bp

Back to Photinia

Pi 0.0121

MarkerSeek score 62.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 949
variable_sites: 61
pi: 0.0121
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	CGGAACTTCGCCCTAATCAA	50.0	50.0	58.2	58.0	1009-1054 bp	1.0	87.4
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	CGGAACTTCGCCCTAATCAA	50.0	50.0	58.2	58.0	1008-1053 bp	1.0	87.4
trnK-UUU-rps16_p3	CCGCACTTAAAAGCCGAGTA	CGGAACTTCGCCCTAATCAA	50.0	50.0	58.3	58.0	1018-1063 bp	1.0	86.5
trnK-UUU-rps16_p4	AAAGCCGAGTACTCTACCGT	TTTTTACGGAACTTCGCCCT	50.0	45.0	58.2	57.7	1015-1060 bp	1.0	85.5
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	TTTTTACGGAACTTCGCCCT	50.0	45.0	58.2	57.7	1014-1059 bp	1.0	85.5