Hotspot

trnH-GUG-psbA

LSC · 189-462 · 274 bp

Back to Photinia

Pi 0.0329

MarkerSeek score 70.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 274
variable_sites: 30
pi: 0.0329
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	375-396 bp	1.0	89.1
trnH-GUG-psbA_p2	ATATTATGGGCGAACGACGG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	386-407 bp	1.0	88.9
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	389-410 bp	1.0	88.9
trnH-GUG-psbA_p4	ATATTATGGGCGAACGACGG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	389-410 bp	1.0	88.8
trnH-GUG-psbA_p5	ATATTATGGGCGAACGACGG	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	436-457 bp	1.0	88.7