Hotspot

ycf1

SSC · 126608-132241 · 5634 bp

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Pi 0.00304

MarkerSeek score 49.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5634
variable_sites: 59
pi: 0.00304
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCAATTTTTCTTTGTTCTGCCA	AGATCCATGAGCTACTTTCT	31.8	40.0	55.3	53.1	178 bp	1.0	42.7
ycf1_p2	TCAATTTTTCTTTGTTCTGCCA	AAGATCCATGAGCTACTTTCT	31.8	38.1	55.3	54.0	179 bp	1.0	42.7
ycf1_p3	TCAATTTTTCTTTGTTCTGCCAA	AGATCCATGAGCTACTTTCT	30.4	40.0	56.0	53.1	178 bp	1.0	42.7
ycf1_p4	TCAATTTTTCTTTGTTCTGCCAA	AAGATCCATGAGCTACTTTCT	30.4	38.1	56.0	54.0	179 bp	1.0	42.7
ycf1_p5	ATCAATTTTTCTTTGTTCTGCCA	AGATCCATGAGCTACTTTCT	30.4	40.0	55.8	53.1	179 bp	1.0	42.7