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psaC-ndhE

SSC · 119828-120519 · 692 bp

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Pi 0.00733

MarkerSeek score 61.20

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 692
variable_sites: 16
pi: 0.00733
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACATCCGTCCCAAGGTATCA	GCTATTGGGCTGGCTATTGT	50.0	50.0	58.1	58.0	877-907 bp	1.0	81.9
psaC-ndhE_p2	AATACATCTGTTGGGCAGGC	GCTATTGGGCTGGCTATTGT	50.0	50.0	58.2	58.0	852-882 bp	1.0	81.1
psaC-ndhE_p3	CAGGCTCGTACACATTGAGT	GCTATTGGGCTGGCTATTGT	50.0	50.0	57.6	58.0	837-867 bp	1.0	80.0
psaC-ndhE_p4	ACATCCGTCCCAAGGTATCA	AGCTATTGGGCTGGCTATTG	50.0	50.0	58.1	57.7	878-908 bp	1.0	79.7
psaC-ndhE_p5	AGCTTTACATCCGTCCCAAG	GCTATTGGGCTGGCTATTGT	50.0	50.0	57.6	58.0	883-913 bp	1.0	79.6