Hotspot

rpl32-trnL-UAG

SSC · 115881-116616 · 736 bp

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Pi 0.0137

MarkerSeek score 56.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 736
variable_sites: 30
pi: 0.0137
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ATTGGCCGAAAACCCCATAG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.2	58.0	1322-1435 bp	1.0	84.3
rpl32-trnL-UAG_p2	ATTGGCCGAAAACCCCATAG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.2	58.0	1323-1436 bp	1.0	84.3
rpl32-trnL-UAG_p3	GATTGGCCGAAAACCCCATA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.2	58.0	1323-1436 bp	1.0	84.3
rpl32-trnL-UAG_p4	GATTGGCCGAAAACCCCATA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.2	58.0	1324-1437 bp	1.0	84.2
rpl32-trnL-UAG_p5	ATTGGCCGAAAACCCCATAG	TCCGCATAAAAGAGCTGCAT	50.0	45.0	58.2	58.0	1909-2022 bp	1.0	83.6