Hotspot

rpl22-rps19

IRb · 85926-86058 · 133 bp

Back to Phoenix

Pi 0.0272

MarkerSeek score 60.10

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 133
variable_sites: 12
pi: 0.0272
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CTTCTCTACCTGAACTCTGGTT	TCTGACTTTCGCGAGACATG	45.5	50.0	56.9	58.0	213-253 bp	1.0	58.9
rpl22-rps19_p2	TCTTCTCTACCTGAACTCTGG	TCTGACTTTCGCGAGACATG	47.6	50.0	55.8	58.0	214-254 bp	1.0	58.7
rpl22-rps19_p3	TCTCTACCTGAACTCTGGTT	TCTGACTTTCGCGAGACATG	45.0	50.0	54.8	58.0	211-251 bp	1.0	58.5
rpl22-rps19_p4	TCTTCTCTACCTGAACTCTGGTT	TCTGACTTTCGCGAGACATG	43.5	50.0	58.3	58.0	214-254 bp	1.0	58.0
rpl22-rps19_p5	CTTCTCTACCTGAACTCTGGTT	CCTACTCTGACTTTCGCGAG	45.5	55.0	56.9	57.8	218-258 bp	1.0	57.5