Hotspot

atpH-atpI

LSC · 14579-15404 · 826 bp

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Pi 0.00161

MarkerSeek score 58.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 826
variable_sites: 3
pi: 0.00161
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.0	57.4	860-899 bp	1.0	78.2
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	ATTTTTGCAACGTTAGCCGC	45.0	45.0	58.0	58.6	888-927 bp	1.0	78.1
atpH-atpI_p3	TACCTTCTACAGCTTGGCCT	TAGGTGAATCCATGGAGGGT	50.0	50.0	58.0	57.4	943-982 bp	1.0	77.9
atpH-atpI_p4	TACCTTCTACAGCTTGGCCT	ATTTTTGCAACGTTAGCCGC	50.0	45.0	58.0	58.6	971-1010 bp	1.0	77.8
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	ATTTTTGCAACGTTAGCCGC	50.0	45.0	57.9	58.6	944-983 bp	1.0	77.4