Hotspot

matK-trnK-UUU

LSC · 3279-4087 · 809 bp

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Pi 0.00402
MarkerSeek score 58.50
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
809
variable_sites
8
pi
0.00402
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 AGAAAGTGTTGTTGCCGAGA AACGGTAGAGTACTCGGCTT 45.0 50.0 57.7 58.2 879-889 bp 1.0 77.8
matK-trnK-UUU_p2 AGAAAGTGTTGTTGCCGAGA ACGGTAGAGTACTCGGCTTT 45.0 50.0 57.7 58.2 878-888 bp 1.0 77.8
matK-trnK-UUU_p3 AGAAAGTGTTGTTGCCGAGA GTCGTGCAGTGCAAATCAAT 45.0 45.0 57.7 57.7 965-975 bp 1.0 76.3
matK-trnK-UUU_p4 AAGTGTTGTTGCCGAGATCT AACGGTAGAGTACTCGGCTT 45.0 50.0 57.4 58.2 876-886 bp 1.0 76.0
matK-trnK-UUU_p5 AAGTGTTGTTGCCGAGATCT ACGGTAGAGTACTCGGCTTT 45.0 50.0 57.4 58.2 875-885 bp 1.0 76.0