Hotspot

ycf2

Genome · 90048-96905 · 6858 bp

Pi 0.00185

MarkerSeek score 49.90

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 6858
variable_sites: 76
pi: 0.00185
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	ATCCGATCCATCCGTTCCTA	CGGACGGCACATATTTCTCA	50.0	50.0	57.7	58.1	215 bp	0.952381	79.6
ycf2_p2	TCCGATCCATCCGTTCCTAT	CGGACGGCACATATTTCTCA	50.0	50.0	57.7	58.1	214 bp	0.952381	79.6
ycf2_p3	ATCCGATCCATCCGTTCCTA	TTTCCGGACGGCACATATTT	50.0	45.0	57.7	57.9	219 bp	0.952381	79.1
ycf2_p4	TCCGATCCATCCGTTCCTAT	TTTCCGGACGGCACATATTT	50.0	45.0	57.7	57.9	218 bp	0.952381	79.1
ycf2_p5	AATTCGATCCGATCCATCCG	CGGACGGCACATATTTCTCA	50.0	50.0	57.6	58.1	221 bp	0.952381	79.0