Hotspot

ndhF

Genome · 113858-116086 · 2229 bp

Back to Phoebe

Pi 0.00256
MarkerSeek score 35.50
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
Genome
length_bp
2229
variable_sites
17
pi
0.00256
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
35.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 ACCAAGAAGATAAGATCTAGTCACT CCGGAAGAAATAAGGGCTGG 36.0 55.0 56.8 58.3 2334 bp 0.857143 35.3
ndhF_p2 ACCAAGAAGATAAGATCTAGTCACT ATAAGGGCTGGTGAATGGGA 36.0 50.0 56.8 58.4 2325 bp 0.857143 35.3
ndhF_p3 ACCAAGAAGATAAGATCTAGTCACT CGGAAGAAATAAGGGCTGGT 36.0 50.0 56.8 57.6 2333 bp 0.857143 35.3
ndhF_p4 ACCAAGAAGATAAGATCTAGTCACT AATAAGGGCTGGTGAATGGG 36.0 50.0 56.8 57.5 2326 bp 0.857143 35.3
ndhF_p5 ACCAAGAAGATAAGATCTAGTCACT TAAGGGCTGGTGAATGGGAA 36.0 50.0 56.8 58.6 2324 bp 0.857143 35.3