Hotspot

rpl20-rps12

Genome · 72612-73368 · 757 bp

Back to Phoebe

Pi 0.00188
MarkerSeek score 52.60
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
Genome
length_bp
757
variable_sites
14
pi
0.00188
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 CTTGAATGAGCCCCTCGAAA AAATGTCACGAAATCCCCCG 50.0 50.0 57.9 58.5 909-913 bp 1.0 76.3
rpl20-rps12_p2 TTGAATGAGCCCCTCGAAAG AAATGTCACGAAATCCCCCG 50.0 50.0 57.9 58.5 908-912 bp 1.0 76.3
rpl20-rps12_p3 CTTGAATGAGCCCCTCGAAA CAGCGTCGAGGAACATGTAC 50.0 55.0 57.9 58.7 872-876 bp 1.0 74.9
rpl20-rps12_p4 TTGAATGAGCCCCTCGAAAG CAGCGTCGAGGAACATGTAC 50.0 55.0 57.9 58.7 871-875 bp 1.0 74.9
rpl20-rps12_p5 TTTGTTCTACGTCTCCGAGC AAATGTCACGAAATCCCCCG 50.0 50.0 57.7 58.5 867-871 bp 1.0 74.6