Hotspot

psbE-petL

Genome · 68332-69574 · 1243 bp

Back to Phoebe

Pi 0.00195
MarkerSeek score 52.20
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
Genome
length_bp
1243
variable_sites
12
pi
0.00195
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA AAGCAGCCAGTAGAAAACCG 50.0 50.0 57.9 58.5 1425-1448 bp 1.0 76.7
psbE-petL_p2 CAACCCGCAATGAATAGGGA GCAGCCAGTAGAAAACCGAA 50.0 50.0 57.9 58.5 1423-1446 bp 1.0 76.6
psbE-petL_p3 ACGAATAACCAACCCGCAAT AAGCAGCCAGTAGAAAACCG 45.0 50.0 58.2 58.5 1434-1457 bp 1.0 75.8
psbE-petL_p4 ACGAATAACCAACCCGCAAT GCAGCCAGTAGAAAACCGAA 45.0 50.0 58.2 58.5 1432-1455 bp 1.0 75.7
psbE-petL_p5 TGCTGACGAATAACCAACCC AAGCAGCCAGTAGAAAACCG 50.0 50.0 58.2 58.5 1439-1462 bp 1.0 75.7