Hotspot

ndhC-trnV-UAC

Genome · 52668-54521 · 1854 bp

Back to Phoebe

Pi 0.00254
MarkerSeek score 51.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
Genome
length_bp
1854
variable_sites
21
pi
0.00254
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTTCACTAATCGGGGCCAAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1995-2010 bp 1.0 78.3
ndhC-trnV-UAC_p2 ACTAATCGGGGCCAAAACTC AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1991-2006 bp 1.0 78.3
ndhC-trnV-UAC_p3 CACTAATCGGGGCCAAAACT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.2 57.8 1992-2007 bp 1.0 78.0
ndhC-trnV-UAC_p4 TCACTAATCGGGGCCAAAAC AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.2 57.8 1993-2008 bp 1.0 77.9
ndhC-trnV-UAC_p5 CTTCACTAATCGGGGCCAAA ACGCATGTTGGGTCTTTGAA 50.0 45.0 57.9 58.3 2061-2076 bp 1.0 77.4