Hotspot

psbM-trnD-GUC

Genome · 31368-32360 · 993 bp

Back to Phoebe

Pi 0.00247
MarkerSeek score 54.40
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
Genome
length_bp
993
variable_sites
14
pi
0.00247
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAACAACGCAGTAGCA AGTTCAATCGGTCAGAGCAC 42.9 50.0 58.2 57.9 1084-1129 bp 1.0 73.3
psbM-trnD-GUC_p2 GAATGAACAACGCAGTAGCA AGTTCAATCGGTCAGAGCAC 45.0 50.0 56.7 57.9 1083-1128 bp 1.0 73.2
psbM-trnD-GUC_p3 GAATGAACAACGCAGTAGCAA AGTTCAATCGGTCAGAGCAC 42.9 50.0 57.5 57.9 1083-1128 bp 1.0 70.9
psbM-trnD-GUC_p4 AGAATGAACAACGCAGTAGCA ATGCTGTACACCCCATTTCC 42.9 50.0 58.2 57.9 1114-1158 bp 0.952381 70.6
psbM-trnD-GUC_p5 GAATGAACAACGCAGTAGCA ATGCTGTACACCCCATTTCC 45.0 50.0 56.7 57.9 1113-1157 bp 0.952381 70.5