Hotspot

petN-psbM

Genome · 30201-31262 · 1062 bp

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Pi 0.00365
MarkerSeek score 51.10
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
Genome
length_bp
1062
variable_sites
20
pi
0.00365
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCTTGGGCT TGCTACTGCGTTGTTCATTCT 50.0 42.9 58.2 58.2 1224-1225 bp 1.0 70.3
petN-psbM_p2 TAGTAAGTCTCGCTTGGGCT TGCTACTGCGTTGTTCATTC 50.0 45.0 58.2 56.7 1224-1225 bp 1.0 70.2
petN-psbM_p3 GGGACACGATTCACATGGAT TGCTACTGCGTTGTTCATTCT 50.0 42.9 57.7 58.2 1245-1246 bp 1.0 68.9
petN-psbM_p4 GGGACACGATTCACATGGAT TGCTACTGCGTTGTTCATTC 50.0 45.0 57.7 56.7 1245-1246 bp 1.0 68.8
petN-psbM_p5 ATGGGGAAGAAGTGGACTCT TGCTACTGCGTTGTTCATTCT 50.0 42.9 57.6 58.2 1163-1164 bp 1.0 68.7