Hotspot

ycf1

SSC · 121734-127226 · 5493 bp

Back to Phlomoides

Pi 0.0139

MarkerSeek score 58.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5493
variable_sites: 571
pi: 0.0139
unique_haplotype_count: 45
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGCAATTGAAAAAGCAAATAACG	AAGCATCGAAATCAAGAAGA	33.3	35.0	58.1	52.9	186 bp	0.978723	48.4
ycf1_p2	TCGCAATTGAAAAAGCAAATAACG	AGCATCGAAATCAAGAAGAA	33.3	35.0	58.1	52.9	185 bp	0.957447	47.2
ycf1_p3	CGCAATTGAAAAAGCAAATAACG	AGCATCGAAATCAAGAAGAA	34.8	35.0	56.8	52.9	184 bp	0.957447	47.2
ycf1_p4	TCGCAATTGAAAAAGCAAATAACG	AGCATCAAATAAAAAGCATCGA	33.3	31.8	58.1	55.1	199 bp	0.957447	46.3
ycf1_p5	CGCAATTGAAAAAGCAAATAACG	AGCATCAAATAAAAAGCATCGA	34.8	31.8	56.8	55.1	198 bp	0.957447	46.3