Hotspot

ndhF-rpl32

SSC · 110975-111479 · 505 bp

Back to Phlomoides

Pi 0.0165
MarkerSeek score 64.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
505
variable_sites
73
pi
0.0165
unique_haplotype_count
38
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CACGCATATTGATATATCTGTTCCA TGATGCAGAAGTACGTTTTT 36.0 35.0 57.2 53.5 479-580 bp 0.978723 51.1
ndhF-rpl32_p2 CACGCATATTGATATATCTGTTCCA TTGATGCAGAAGTACGTTTT 36.0 35.0 57.2 53.5 480-581 bp 0.978723 51.1
ndhF-rpl32_p3 CACGCATATTGATATATCTGTTCCA TTTGATGCAGAAGTACGTTT 36.0 35.0 57.2 53.5 481-582 bp 0.978723 51.1
ndhF-rpl32_p4 CACGCATATTGATATATCTGTTCCA TTTTGATGCAGAAGTACGTT 36.0 35.0 57.2 53.5 482-583 bp 0.978723 51.1
ndhF-rpl32_p5 CACGCATATTGATATATCTGTTCCA TTTTTGATGCAGAAGTACGT 36.0 35.0 57.2 53.5 483-584 bp 0.978723 51.1