Hotspot

ycf4-cemA

LSC · 60191-60749 · 559 bp

Back to Phlomoides

Pi 0.0109

MarkerSeek score 68.90

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 559
variable_sites: 58
pi: 0.0109
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CTTGCGCGTACCAATTGAAG	GAAATCCACCAGGGCAGAAA	50.0	50.0	58.1	58.1	443-667 bp	1.0	89.8
ycf4-cemA_p2	CGTTGACCCGTACTGATGAG	GAAATCCACCAGGGCAGAAA	55.0	50.0	58.1	58.1	513-737 bp	1.0	89.5
ycf4-cemA_p3	GAACAAAAAGCCGCGGAATT	GAAATCCACCAGGGCAGAAA	45.0	50.0	58.2	58.1	472-696 bp	1.0	88.6
ycf4-cemA_p4	TTTCTTGCGCGTACCAATTG	GAAATCCACCAGGGCAGAAA	45.0	50.0	57.7	58.1	446-670 bp	1.0	88.1
ycf4-cemA_p5	CGTTGACCCGTACTGATGAG	TCCACCAGGGCAGAAATACT	55.0	50.0	58.1	58.3	509-733 bp	1.0	87.5