Hotspot

ndhC-trnV-UAC

LSC · 50014-50967 · 954 bp

Back to Phlomoides

Pi 0.0134

MarkerSeek score 64.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 954
variable_sites: 75
pi: 0.0134
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAATCCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1075-1106 bp	1.0	84.5
ndhC-trnV-UAC_p2	TTCTCCGGCCCTTTCCTAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	1096-1127 bp	1.0	81.9
ndhC-trnV-UAC_p3	GGGGCTAAAATCCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1074-1105 bp	1.0	81.8
ndhC-trnV-UAC_p4	GGGGCTAAAATCCCGGAAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1063-1094 bp	1.0	80.7
ndhC-trnV-UAC_p5	CCCTTTCCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.6	57.8	1088-1119 bp	1.0	80.6