Hotspot

trnT-GGU-psbD

LSC · 31530-32776 · 1247 bp

Back to Phlomoides

Pi 0.00903

MarkerSeek score 65.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1247
variable_sites: 101
pi: 0.00903
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1342-1420 bp	1.0	87.4
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1345-1423 bp	1.0	87.2
trnT-GGU-psbD_p3	ATAGTGACTCGTCCGGGAAT	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1388-1466 bp	0.978723	86.6
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTC	55.0	50.0	58.1	57.6	1341-1419 bp	0.957447	84.1
trnT-GGU-psbD_p5	ATAGTGACTCGTCCGGGAAT	CAAAAACGAAACGGTCCCTC	50.0	50.0	57.9	57.6	1387-1465 bp	0.93617	83.4