Hotspot

rpoB-trnC-GCA

LSC · 26952-28047 · 1096 bp

Back to Phlomoides

Pi 0.011

MarkerSeek score 65.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1096
variable_sites: 98
pi: 0.011
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CATTGCCTCATTTGCATCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1094-1209 bp	1.0	88.1
rpoB-trnC-GCA_p2	CATTGCCTCATTTGCATCCC	TTTGGTAATGAGGCGACACC	50.0	50.0	57.8	58.2	1113-1228 bp	1.0	87.3
rpoB-trnC-GCA_p3	CATTGCCTCATTTGCATCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.8	58.4	1076-1191 bp	1.0	86.1
rpoB-trnC-GCA_p4	ATTGCCTCATTTGCATCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.9	58.1	1093-1208 bp	1.0	83.0
rpoB-trnC-GCA_p5	ACATTGCCTCATTTGCATCC	CCGGATTTGAACTGGGGAAA	45.0	50.0	57.0	58.1	1095-1210 bp	1.0	82.2