Hotspot

trnK-UUU-rps16

LSC · 4184-5097 · 914 bp

Back to Phlomoides

Pi 0.0124
MarkerSeek score 66.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
914
variable_sites
95
pi
0.0124
unique_haplotype_count
40
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AGGAACTTCCGCCTAATCAA 50.0 45.0 58.2 56.5 970-1029 bp 1.0 78.6
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AGGAACTTCCGCCTAATCAA 50.0 45.0 58.2 56.5 969-1028 bp 1.0 78.6
trnK-UUU-rps16_p3 TCTAGCCGCACTTAAAAGCC AGGAACTTCCGCCTAATCAA 50.0 45.0 58.3 56.5 984-1043 bp 1.0 77.7
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA AGGAACTTCCGCCTAATCAA 50.0 45.0 58.3 56.5 979-1038 bp 1.0 77.6
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AGGAACTTCCGCCTAATCAAA 50.0 42.9 58.2 57.3 969-1028 bp 1.0 76.7