Hotspot

trnH-GUG-psbA

LSC · 76-305 · 230 bp

Back to Phlomoides

Pi 0.0274

MarkerSeek score 74.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 230
variable_sites: 39
pi: 0.0274
unique_haplotype_count: 33
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CAACCGTGCTAACCTTGGTA	55.0	50.0	58.1	57.8	320-372 bp	1.0	89.6
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	334-386 bp	1.0	89.6
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	333-385 bp	1.0	89.5
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	335-387 bp	1.0	89.5
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	ATCAACCGTGCTAACCTTGG	55.0	50.0	58.1	58.2	322-374 bp	1.0	89.5