Hotspot

rps3

LSC · 107163-107825 · 663 bp

Back to Phlomis

Pi 0.00302

MarkerSeek score 60.50

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 663
variable_sites: 2
pi: 0.00302
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	CCCCTTTTTGTTCTATCGACG	ACCCTAGATGGCAAAAGACA	47.6	45.0	57.0	56.4	762 bp	1.0	51.6
rps3_p2	CCCTTTTTGTTCTATCGACGG	ACCCTAGATGGCAAAAGACA	47.6	45.0	57.0	56.4	761 bp	1.0	51.6
rps3_p3	TGAGTTTCCCCCTTTTTGTTCT	ACCCTAGATGGCAAAAGACA	40.9	45.0	58.0	56.4	770 bp	1.0	51.5
rps3_p4	CGGAATTTAGTTGATTGGCCAG	ACCCTAGATGGCAAAAGACA	45.5	45.0	58.0	56.4	804 bp	1.0	51.4
rps3_p5	TCCCCCTTTTTGTTCTATCGAC	ACCCTAGATGGCAAAAGACA	45.5	45.0	58.1	56.4	764 bp	1.0	51.2