Hotspot

rps16

LSC · 30561-31680 · 1120 bp

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Pi 0.00179

MarkerSeek score 59.90

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1120
variable_sites: 2
pi: 0.00179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ATATACAAGTCGCTACCCCC	TATGGACAGCTGAACCAGTG	50.0	50.0	56.2	57.2	1249 bp	1.0	59.6
rps16_p2	ATATACAAGTCGCTACCCCC	ATGGACAGCTGAACCAGTGA	50.0	50.0	56.2	58.9	1248 bp	1.0	58.2
rps16_p3	ATATACAAGTCGCTACCCCC	GTCTATGGACAGCTGAACCA	50.0	50.0	56.2	56.9	1252 bp	1.0	57.2
rps16_p4	ATATACAAGTCGCTACCCCC	TGTCTATGGACAGCTGAACC	50.0	50.0	56.2	56.9	1253 bp	1.0	57.2
rps16_p5	ATATACAAGTCGCTACCCCC	CTATGGACAGCTGAACCAGT	50.0	50.0	56.2	56.9	1250 bp	1.0	57.2