Hotspot

rpl22

LSC · 107810-108274 · 465 bp

Back to Phlomis

Pi 0.00215

MarkerSeek score 50.70

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 465
variable_sites: 1
pi: 0.00215
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CAAGTCGGAAACCAAGTGGA	TGAATTTCCAAGGACACGCA	50.0	45.0	58.0	58.0	592 bp	1.0	79.6
rpl22_p2	GATGGCTTTGGGTTGTACCA	TGAATTTCCAAGGACACGCA	50.0	45.0	58.1	58.0	610 bp	1.0	79.3
rpl22_p3	TGATGGCTTTGGGTTGTACC	TGAATTTCCAAGGACACGCA	50.0	45.0	58.1	58.0	611 bp	1.0	79.3
rpl22_p4	AAGGGAATGATGGCTTTGGG	TGAATTTCCAAGGACACGCA	50.0	45.0	58.1	58.0	618 bp	1.0	78.9
rpl22_p5	GGGTTGTACCAAGTCGGAAA	TGAATTTCCAAGGACACGCA	50.0	45.0	57.7	58.0	601 bp	1.0	77.9