Hotspot

rpl16

LSC · 105704-107007 · 1304 bp

Back to Phlomis

Pi 0.0023

MarkerSeek score 46.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1304
variable_sites: 3
pi: 0.0023
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ATTAATTTTCGAGCCCCGCT	TTCCCCTACAAACCATTCGC	45.0	50.0	57.9	58.2	1757 bp	1.0	78.4
rpl16_p2	ACCGCTGTCACGTTTAAGTT	TTCCCCTACAAACCATTCGC	45.0	50.0	58.1	58.2	1920 bp	1.0	78.4
rpl16_p3	ATTAATTTTCGAGCCCCGCT	AATCGAAAGTAGACCGCGAG	45.0	50.0	57.9	57.8	2056 bp	1.0	78.2
rpl16_p4	ATTAATTTTCGAGCCCCGCT	GGAATTGCCCGCATAGAGAT	45.0	50.0	57.9	57.8	2133 bp	1.0	78.2
rpl16_p5	ACCGCTGTCACGTTTAAGTT	AATCGAAAGTAGACCGCGAG	45.0	50.0	58.1	57.8	2219 bp	1.0	78.2