Hotspot

ndhF

SSC · 134182-136404 · 2223 bp

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Pi 0.00495

MarkerSeek score 60.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 11
pi: 0.00495
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGACCCGTTTCTGAAGAGTC	AGGTCGGGTGAATTGGAAAC	50.0	50.0	56.9	58.1	2354-2355 bp	1.0	70.8
ndhF_p2	GACCCGTTTCTGAAGAGTCT	AGGTCGGGTGAATTGGAAAC	50.0	50.0	56.9	58.1	2353-2354 bp	1.0	70.8
ndhF_p3	AGACCCGTTTCTGAAGAGTC	GAAGGTCGGGTGAATTGGAA	50.0	50.0	56.9	57.8	2356-2357 bp	1.0	70.0
ndhF_p4	GACCCGTTTCTGAAGAGTCT	GAAGGTCGGGTGAATTGGAA	50.0	50.0	56.9	57.8	2355-2356 bp	1.0	70.0
ndhF_p5	GACCCGTTTCTGAAGAGTCTT	AGGTCGGGTGAATTGGAAAC	47.6	50.0	57.6	58.1	2353-2354 bp	1.0	68.5