Hotspot

ccsA

SSC · 137741-138712 · 972 bp

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Pi 0.00206

MarkerSeek score 59.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 2
pi: 0.00206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGCGGCATAGCATCTCTTAA	TATGATTCGACAGGCTCTCG	50.0	50.0	57.8	56.9	1170-1171 bp	1.0	70.3
ccsA_p2	GGCGGCATAGCATCTCTTAA	ATATGATTCGACAGGCTCTCG	50.0	47.6	57.8	57.4	1171-1172 bp	1.0	66.3
ccsA_p3	TGGCGGCATAGCATCTCTTA	TATGATTCGACAGGCTCTCG	50.0	50.0	59.0	56.9	1171-1172 bp	1.0	64.0
ccsA_p4	GGCGGCATAGCATCTCTTAA	ATGATTCGACAGGCTCTCG	50.0	52.6	57.8	57.0	1169-1170 bp	1.0	63.2
ccsA_p5	GGCGGCATAGCATCTCTTAA	GATTCGACAGGCTCTCGC	50.0	61.1	57.8	58.0	1167-1168 bp	1.0	62.8