Hotspot

trnT-GGU-psbD

LSC · 56995-58183 · 1189 bp

Back to Phlomis

Pi 0.00252

MarkerSeek score 60.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1189
variable_sites: 3
pi: 0.00252
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1349-1350 bp	1.0	78.8
trnT-GGU-psbD_p2	AGTGACTCGTCCTGGAATGA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1393-1394 bp	1.0	78.7
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1352-1353 bp	1.0	78.6
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1339-1340 bp	1.0	77.7
trnT-GGU-psbD_p5	AGTGACTCGTCCTGGAATGA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1383-1384 bp	1.0	77.6