Hotspot

atpH-atpI

LSC · 39053-40038 · 986 bp

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Pi 0.00206

MarkerSeek score 61.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 986
variable_sites: 2
pi: 0.00206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.0	57.9	1104-1120 bp	1.0	80.0
atpH-atpI_p2	AGCCAATCCAGCAGCAATAA	CCCGTCATGTTCCTTGGATT	45.0	50.0	57.9	57.9	1120-1136 bp	1.0	79.1
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.2	57.9	1105-1121 bp	1.0	78.8
atpH-atpI_p4	GCCAATCCAGCAGCAATAAC	CCCGTCATGTTCCTTGGATT	50.0	50.0	57.8	57.9	1119-1135 bp	1.0	78.5
atpH-atpI_p5	TCCAGCAGCAATAACGGAAG	CCCGTCATGTTCCTTGGATT	50.0	50.0	58.3	57.9	1114-1130 bp	1.0	78.2