Hotspot

trnS-GCU-trnS-CGA

LSC · 33964-34530 · 567 bp

Back to Phlomis

Pi 0.00353
MarkerSeek score 60.10
Primer available yes
Label trnS-GCU-trnS-CGA

Feature Metadata

label_name
trnS-GCU-trnS-CGA
feature_type
igs
region
LSC
length_bp
567
variable_sites
2
pi
0.00353
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnS-CGA_p1 CAATCCGACGCTTTAGTCCA ACCACTAAACTATACCCGCT 50.0 45.0 58.0 55.6 622 bp 1.0 61.4
trnS-GCU-trnS-CGA_p2 AATCCGACGCTTTAGTCCAC ACCACTAAACTATACCCGCT 50.0 45.0 58.0 55.6 621 bp 1.0 61.4
trnS-GCU-trnS-CGA_p3 ACGGAAAGAGAGGGATTCGA ACCACTAAACTATACCCGCT 50.0 45.0 58.2 55.6 676 bp 1.0 60.2
trnS-GCU-trnS-CGA_p4 AGCAATCCGACGCTTTAGTC ACCACTAAACTATACCCGCT 50.0 45.0 58.4 55.6 624 bp 1.0 58.6
trnS-GCU-trnS-CGA_p5 CAATCCGACGCTTTAGTCCA CGAACGAATCACACTTTTACCA 50.0 40.9 58.0 57.3 640 bp 1.0 58.5