Hotspot

trnK-UUU-rps16

LSC · 29781-30560 · 780 bp

Back to Phlomis

Pi 0.00257

MarkerSeek score 59.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 780
variable_sites: 2
pi: 0.00257
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.2	58.0	902-921 bp	1.0	78.9
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.2	58.0	901-920 bp	1.0	78.9
trnK-UUU-rps16_p3	TCTAGCCGCACTTAAAAGCC	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.3	58.0	916-935 bp	1.0	78.1
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.3	58.0	911-930 bp	1.0	78.0
trnK-UUU-rps16_p5	TACCGTTGAGTTAGCAACCC	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	57.5	58.0	888-907 bp	1.0	76.5