Hotspot

atpB

LSC · 51567-53063 · 1497 bp

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Pi 0.00122
MarkerSeek score 50.20
Primer available yes
Label atpB

Feature Metadata

label_name
atpB
feature_type
gene
region
LSC
length_bp
1497
variable_sites
3
pi
0.00122
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB_p1 TGTTAATGGGGGCATGGTTT TTTTTGGATTTGATTTGGCA 45.0 30.0 57.7 52.3 1698 bp 1.0 40.1
atpB_p2 TTAATGGGGGCATGGTTTGG TTTTTGGATTTGATTTGGCA 50.0 30.0 58.4 52.3 1696 bp 1.0 40.1
atpB_p3 GGGGGCATGGTTTGGTAATA TTTTTGGATTTGATTTGGCA 50.0 30.0 57.5 52.3 1691 bp 1.0 40.1
atpB_p4 GCATGGTTTGGTAATACGCC TTTTTGGATTTGATTTGGCA 50.0 30.0 57.5 52.3 1687 bp 1.0 40.1
atpB_p5 GGCATGGTTTGGTAATACGC TTTTTGGATTTGATTTGGCA 50.0 30.0 57.5 52.3 1688 bp 1.0 40.1