Hotspot

ndhF-rpl32

SSC · 103694-104536 · 843 bp

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Pi 0.00378
MarkerSeek score 52.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
843
variable_sites
5
pi
0.00378
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TGCTAAAGAATAAGCGTGTACT 55.0 36.4 58.1 55.6 997-998 bp 1.0 44.6
ndhF-rpl32_p2 GGCCAAACCCCATTGACATA TGCTAAAGAATAAGCGTGTACT 50.0 36.4 58.1 55.6 1023-1024 bp 1.0 44.3
ndhF-rpl32_p3 AAAAGGCCAAACCCCATTGA TGCTAAAGAATAAGCGTGTACT 45.0 36.4 58.2 55.6 1027-1028 bp 1.0 43.9
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTGCTAAAGAATAAGCGTGTACT 55.0 34.8 58.1 56.3 998-999 bp 1.0 42.3
ndhF-rpl32_p5 GGCCAAACCCCATTGACATA TTGCTAAAGAATAAGCGTGTACT 50.0 34.8 58.1 56.3 1024-1025 bp 1.0 42.1