Hotspot

rps11-rpl36

LSC · 75405-75589 · 185 bp

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Pi 0.00996
MarkerSeek score 59.50
Primer available yes
Label rps11-rpl36

Feature Metadata

label_name
rps11-rpl36
feature_type
igs
region
LSC
length_bp
185
variable_sites
3
pi
0.00996
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11-rpl36_p1 TACGCGCTTTCTTACGTGAA GCGTGGACGAATTAGAGTCA 45.0 50.0 58.0 57.7 287-289 bp 1.0 78.8
rps11-rpl36_p2 TCTACGTGCACTCTTCCGTA GCGTGGACGAATTAGAGTCA 50.0 50.0 58.2 57.7 337-339 bp 1.0 77.5
rps11-rpl36_p3 TACGCGCTTTCTTACGTGAA GGCGTGGACGAATTAGAGTC 45.0 55.0 58.0 58.4 288-290 bp 1.0 77.5
rps11-rpl36_p4 CGTGCACTCTTCCGTAAACT GCGTGGACGAATTAGAGTCA 50.0 50.0 58.2 57.7 333-335 bp 1.0 77.2
rps11-rpl36_p5 ATACGCGCTTTCTTACGTGA GCGTGGACGAATTAGAGTCA 45.0 50.0 57.7 57.7 288-290 bp 1.0 77.1