Hotspot

trnF-GAA-ndhJ

LSC · 47027-47577 · 551 bp

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Pi 0.00212
MarkerSeek score 41.00
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
551
variable_sites
2
pi
0.00212
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA CGCGCCTTAAACGTATCCTA 50.0 50.0 58.1 57.9 686-687 bp 1.0 78.8
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA GAAAGTTGGATAGGGTGGCC 50.0 55.0 58.1 58.2 660-661 bp 1.0 77.9
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA ATGATAATCATCCGCGCCTT 50.0 45.0 58.1 57.5 698-699 bp 1.0 76.0
trnF-GAA-ndhJ_p4 CCTCGTGTCACCAGTTCAAA ATCCGCGCCTTAAACGTATC 50.0 50.0 58.1 58.5 689-690 bp 1.0 75.8
trnF-GAA-ndhJ_p5 CCTCGTGTCACCAGTTCAAA TGAAAGTTGGATAGGGTGGC 50.0 50.0 58.1 57.5 661-662 bp 1.0 75.8