Hotspot

trnC-GCA-rpoB

LSC · 17851-19040 · 1190 bp

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Pi 0.0024
MarkerSeek score 54.50
Primer available yes
Label trnC-GCA-rpoB

Feature Metadata

label_name
trnC-GCA-rpoB
feature_type
igs
region
LSC
length_bp
1190
variable_sites
5
pi
0.0024
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-rpoB_p1 AAAGGATTTGCAGTCCCCTG ATTCCCTCGTTTCCATTCCG 50.0 50.0 58.1 58.0 1250-1259 bp 1.0 80.1
trnC-GCA-rpoB_p2 AAAGGATTTGCAGTCCCCTG TGTGGACATTCCCTCGTTTC 50.0 50.0 58.1 57.8 1257-1266 bp 1.0 79.0
trnC-GCA-rpoB_p3 AAAGGATTTGCAGTCCCCTG TGTCAAGTTCTTCTGCCAGG 50.0 50.0 58.1 57.7 1333-1342 bp 1.0 78.3
trnC-GCA-rpoB_p4 AAAGGATTTGCAGTCCCCTG CCGGGTATTGTGGACATTCC 50.0 55.0 58.1 58.3 1265-1274 bp 1.0 77.8
trnC-GCA-rpoB_p5 AAAGGATTTGCAGTCCCCTG CGGGTATTGTGGACATTCCC 50.0 55.0 58.1 58.3 1264-1273 bp 1.0 77.8