Hotspot

trnK-UUU-rps16

LSC · 3957-4515 · 559 bp

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Pi 0.00119
MarkerSeek score 50.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
559
variable_sites
1
pi
0.00119
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TGGACGAATTTGTTGCTCCA GCTCAACCGACAAGAACTGT 45.0 50.0 58.0 58.4 719-723 bp 1.0 77.0
trnK-UUU-rps16_p2 AGTCGCACTTAAAAGCCGAG GCTCAACCGACAAGAACTGT 50.0 50.0 58.6 58.4 678-682 bp 1.0 72.7
trnK-UUU-rps16_p3 CTGGACGAATTTGTTGCTCC GCTCAACCGACAAGAACTGT 50.0 50.0 57.4 58.4 720-724 bp 1.0 72.3
trnK-UUU-rps16_p4 TGGACGAATTTGTTGCTCCA CGCTCAACCGACAAGAACT 45.0 52.6 58.0 58.1 720-724 bp 1.0 71.6
trnK-UUU-rps16_p5 TCGCACTTAAAAGCCGAGTA GCTCAACCGACAAGAACTGT 45.0 50.0 57.3 58.4 676-680 bp 1.0 71.4