Hotspot

ycf1

Genome · 127366-132075 · 4710 bp

Back to Phlegmariurus

Pi 0.465

MarkerSeek score 73.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 4710
variable_sites: 1779
pi: 0.465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGAAAACCCCTACCCCAATG	GCAATATCGGAGTCACCCTG	55.0	55.0	58.1	58.1	1091 bp	0.5	57.9
ycf1_p2	GGAAAACCCCTACCCCAATG	CGGAGTCACCCTGGATTTTT	55.0	50.0	58.1	57.8	1084 bp	0.5	57.3
ycf1_p3	TGAGCTGATGCAAAATCCCA	GCAATATCGGAGTCACCCTG	45.0	55.0	57.8	58.1	1073 bp	0.5	57.2
ycf1_p4	CCTACCCCAATGAGCTGATG	GCAATATCGGAGTCACCCTG	55.0	55.0	57.7	58.1	1083 bp	0.5	56.8
ycf1_p5	TGAGCTGATGCAAAATCCCA	CGGAGTCACCCTGGATTTTT	45.0	50.0	57.8	57.8	1066 bp	0.5	56.6