Hotspot

ndhH

Genome · 125382-126563 · 1182 bp

Back to Phlegmariurus

Pi 0.5

MarkerSeek score 81.10

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: Genome
length_bp: 1182
variable_sites: 509
pi: 0.5
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GGCTCGAAGACCACCTAAAG	TCCTCCTCGGTTTCTGAAGA	55.0	50.0	58.0	57.7	2028 bp	0.5	57.5
ndhH_p2	ACGAGTCGCACGTAGAGATA	TCCTCCTCGGTTTCTGAAGA	50.0	50.0	58.1	57.7	2100 bp	0.5	57.0
ndhH_p3	CGTGTTCAGGTCCAATACGT	TCCTCCTCGGTTTCTGAAGA	50.0	50.0	57.9	57.7	1735 bp	0.5	57.0
ndhH_p4	CAGCGTGTTCAGGTCCAATA	TCCTCCTCGGTTTCTGAAGA	50.0	50.0	57.9	57.7	1738 bp	0.5	56.8
ndhH_p5	AGCGTGTTCAGGTCCAATAC	TCCTCCTCGGTTTCTGAAGA	50.0	50.0	57.9	57.7	1737 bp	0.5	56.8