Hotspot

ndhG

Genome · 122025-122585 · 561 bp

Back to Phlegmariurus

Pi 0.437

MarkerSeek score 81.10

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: Genome
length_bp: 561
variable_sites: 216
pi: 0.437
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	GGCTGATCCTATTGCTGCTT	GCGGAAACTGTGTCGAGTAT	50.0	50.0	58.0	58.0	1346 bp	0.5	59.8
ndhG_p2	GGCTGATCCTATTGCTGCTT	GCCCTTATCAGTGGTGGAAG	50.0	55.0	58.0	58.0	1231 bp	0.5	59.5
ndhG_p3	ATGGCTGATCCTATTGCTGC	GCGGAAACTGTGTCGAGTAT	50.0	50.0	58.1	58.0	1348 bp	0.5	59.2
ndhG_p4	GGCTGATCCTATTGCTGCTT	AACTGTGTCGAGTATTGCCC	50.0	50.0	58.0	57.9	1341 bp	0.5	59.1
ndhG_p5	GGCTGATCCTATTGCTGCTT	AGCAAACTATACAAGCCGCA	50.0	45.0	58.0	57.9	1628 bp	0.5	59.0