Hotspot

ndhD

Genome · 119352-120824 · 1473 bp

Back to Phlegmariurus

Pi 0.452

MarkerSeek score 83.30

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: Genome
length_bp: 1473
variable_sites: 635
pi: 0.452
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AAAGGCTGGCAGGGTAAAAA	GCTTGTCCCACCGATGTATT	45.0	50.0	57.9	58.0	2256 bp	0.5	58.9
ndhD_p2	TTGGTTACTTGGCTCGCATT	GCTTGTCCCACCGATGTATT	45.0	50.0	58.1	58.0	2304 bp	0.5	58.9
ndhD_p3	TTTGGTTACTTGGCTCGCAT	GCTTGTCCCACCGATGTATT	45.0	50.0	58.1	58.0	2305 bp	0.5	58.9
ndhD_p4	AAAGGCTGGCAGGGTAAAAA	TGGGTCTAGCCTACTAACCG	45.0	55.0	57.9	57.9	2084 bp	0.5	58.7
ndhD_p5	TTGGTTACTTGGCTCGCATT	TGGGTCTAGCCTACTAACCG	45.0	55.0	58.1	57.9	2132 bp	0.5	58.7