Hotspot

ccsA

Genome · 118105-119136 · 1032 bp

Back to Phlegmariurus

Pi 0.458

MarkerSeek score 80.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: Genome
length_bp: 1032
variable_sites: 394
pi: 0.458
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CTTGAGCATCTCGGTTCGAA	CTTCCTGGCAGGAACAAGTT	50.0	50.0	58.0	58.0	1932 bp	0.5	59.9
ccsA_p2	CATCACCCCAAGATGGTACG	CTTCCTGGCAGGAACAAGTT	55.0	50.0	58.0	58.0	2162 bp	0.5	59.5
ccsA_p3	CTTGAGCATCTCGGTTCGAA	TGCATTACCGGGTACAAGTG	50.0	50.0	58.0	57.9	1812 bp	0.5	59.2
ccsA_p4	CTTGAGCATCTCGGTTCGAA	CCTTGCATTACCGGGTACAA	50.0	50.0	58.0	57.9	1815 bp	0.5	59.0
ccsA_p5	CATCACCCCAAGATGGTACG	TGCATTACCGGGTACAAGTG	55.0	50.0	58.0	57.9	2042 bp	0.5	58.8