Hotspot

rpl32-trnL-UAG

Genome · 116799-117839 · 1041 bp

Back to Phlegmariurus

Pi 0.439

MarkerSeek score 81.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: Genome
length_bp: 1041
variable_sites: 393
pi: 0.439
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GTCACTTCGCAACGCTAAAC	TTCGAACCGAGATGCTCAAG	50.0	50.0	58.1	58.0	1697 bp	0.5	59.4
rpl32-trnL-UAG_p2	ATGATGTTCGTCACTTCGCA	TTCGAACCGAGATGCTCAAG	45.0	50.0	57.9	58.0	1706 bp	0.5	59.3
rpl32-trnL-UAG_p3	TGATGTTCGTCACTTCGCAA	TTCGAACCGAGATGCTCAAG	45.0	50.0	58.1	58.0	1705 bp	0.5	58.8
rpl32-trnL-UAG_p4	GTCACTTCGCAACGCTAAAC	AAGCACTGCTTCCTAAGAGC	50.0	50.0	58.1	57.9	1680 bp	0.5	58.6
rpl32-trnL-UAG_p5	ATGATGTTCGTCACTTCGCA	AAGCACTGCTTCCTAAGAGC	45.0	50.0	57.9	57.9	1689 bp	0.5	58.5