Hotspot

trnL-CAA-ndhB

Genome · 96238-97193 · 956 bp

Back to Phlegmariurus

Pi 0.07

MarkerSeek score 79.00

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: Genome
length_bp: 956
variable_sites: 61
pi: 0.07
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	CTCGCGTGTCTACCATTTCA	ACCTATTTTGGTGCGGATGG	50.0	50.0	58.0	58.2	1210-1273 bp	1.0	86.3
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	GCTTCTTGTTTTGCCTCGTG	50.0	50.0	58.0	58.2	1283-1346 bp	1.0	86.2
trnL-CAA-ndhB_p3	CTCGCGTGTCTACCATTTCA	ATTCAGTTCACGCACAGGAA	50.0	45.0	58.0	57.7	1353-1416 bp	1.0	85.9
trnL-CAA-ndhB_p4	CTCGCGTGTCTACCATTTCA	AGCTTCTTGTTTTGCCTCGT	50.0	45.0	58.0	58.3	1284-1347 bp	1.0	85.6
trnL-CAA-ndhB_p5	CTCGCGTGTCTACCATTTCA	TCTTGTTTTGCCTCGTGTCT	50.0	45.0	58.0	57.7	1280-1343 bp	1.0	85.5